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[ Source: pybigwig  ]

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Python 3 module for quick access to bigBed and bigWig files

This is a Python extension, written in C, for quick access to bigBed files, and access to and creation of bigWig files.

The bigWig format was originally created in the context of genome browsers. There, computing exact summary statistics for a given interval is less important than quickly being able to compute an approximate statistic. Because of this, bigWig files contain not only interval-value associations, but also `sum of values`/`sum of squared values`/`minimum value`/`maximum value`/`number of bases covered` for equally sized bins of various sizes. These different sizes are referred to as "zoom levels". The smallest zoom level has bins that are 16 times the mean interval size in the file and each subsequent zoom level has bins 4 times larger than the previous. This methodology is used in Kent's tools and, therefore, likely used in almost every currently existing bigWig file.

When a bigWig file is queried for a summary statistic, the size of the interval is used to determine whether to use a zoom level and, if so, which one. The optimal zoom level is that which has the largest bins no more than half the width of the desired interval. If no such zoom level exists, the original intervals are instead used for the calculation.

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